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Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm ArchiMer
Suquet, Marc; Quere, Claudie; Mingant, Christian; Lebrun, Luc; Ratiskol, Dominique; Miner, Philippe; Cosson, Jacky.
Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (YAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, YAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed...
Tipo: Text Palavras-chave: Spermatozoa; CASA; Maturation; Sperm motility; Bivalve; Pecten maximus.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00157/26852/24983.pdf
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Genetic structure at different spatial scales in the pearl oyster (Pinctada margaritifera cumingii) in French Polynesian lagoons: beware of sampling strategy and genetic patchiness ArchiMer
Arnaud, Sophie; Vonau, Vincent; Rouxel, Catherine; Bonhomme, Francois; Prou, Jean; Goyard, Emmanuel; Boudry, Pierre.
In order to study further the genetic structure of the pearl oyster Pinctada margaritifera in French Polynesia with a special consideration for the sampling scale, we analyzed or re-analyzed sets of data based on nuclear DNA markers obtained at different spatial scales. At a large scale (several 1,000 km), the remote Marquesas Islands were confirmed to be significantly differentiated from Tuamotu-Gambier and Society archipelagos, with a marked difference however for the two main islands that are different from each other. At a medium scale (several 10 to several 100 km), overall homogeneity was observed within and between these two archipelagos, with some exceptions. This could be attributed both to large-scale larval dispersal and to human-driven spat...
Tipo: Text Palavras-chave: Population genetics; Polynesia; Bivalve; Pearl oyster; Benthic organisms; Sampling strategy; Genetic patchiness.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4960.pdf
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Influence of low temperatures on the survival of the Pacific oyster (Crassostrea gigas) infected with Ostreid herpesvirus type 1 ArchiMer
Pernet, Fabrice; Tamayo, David; Petton, Bruno.
Mortalities of oyster seed of Crassostrea gigas associated with ostreid herpes virus OsHV-1 μVar have been observed in many oyster producing countries since 2008. The objective of this study was to further investigate whether low temperature treatments can offer a viable option to mitigate oyster mortalities. An experiment was set-up to further evaluate the effect of low temperature treatments (10 and 13°C vs. 21°C) and their duration (6 d to 83 d) on the survival of oysters previously infected with OsHV-1 μVar by means of exposure to field conditions in areas where mortalities were occurring. Average survival of oysters infected with OsHV-1μVar was 71% after 83 d at low temperatures compared to only 23% in controls maintained at 21°C. During...
Tipo: Text Palavras-chave: Bivalve; Disease; OsHV-1; Oyster; Temperature; Virus.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00259/37047/35555.pdf
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Relations entre le contenu lipidique des ovocytes de Pecten maximus (Mollusque, bivalve) et les performances des larves en élevage ArchiMer
Le Pennec, Marcel; Gueguen,.
The lipid content of spawned oocytes of Pecten maximus was studied by image analysis of semi -thin histological sections and a lipid index was defined. The female gametes were obtained by thermic stimulations of mature adults scallops from different populations after out of season conditioning or sampled in situ during the spawning season. After fertilization, the larval development was followed. The results reveal a large variability in lipid content of normal oocytes. The lipid content can be regarded as homogeneous inside a spawning but the heterogeneity is greater for wild scallops than for conditioned ones. A strong relationship appears between the lipid index and the hatching success, and the number of abnormal larvae after two days rearing. But no...
Tipo: Text Palavras-chave: Mollusques; Bivalves; Pecten maximus; Ovocytes; Lipides; Elevages larvaires; Mollusc; Bivalve; Pecten maximus; Oocytes; Lipids; Larval development.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00022/13345/10379.pdf
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Evidence for interspecies transmission of oyster herpesvirus in marine bivalves ArchiMer
Arzul, Isabelle; Renault, Tristan; Lipart, Cécile; Davison, Andrew.
Since 1991, numerous herpesvirus infections associated with high mortality have been reported around the world in various marine bivalve species. In order to determine whether these infections are due to ostreid herpesvirus-l (OsHV1), a previously characterized pathogen of the Japanese oyster (Crassostrea gigas), PCR analysis was carried out on 30 samples of larvae collected from four bivalve species (C. gigas, Ostrea edulis, Ruditapes decussatus and Ruditapes philippinarum), most exhibiting mortality prior to collection. All samples were shown to be infected by OsHV1. Viral genomes in three samples of C. gigas and three of R. philippinarum that originated from the same hatchery were unusual in bearing a deletion of at least 2.8 kbp in an inverted repeat...
Tipo: Text Palavras-chave: Bivalve; Crassostrea gigas; Herpesvirus; Japanese oyster; Transmission.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-705.pdf
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Impact of environmental variability on Pinctada margaritifera life-history traits: A full life cycle deb modeling approach ArchiMer
Sangare, Nathanael; Lo-yat, Alain; Le Moullac, Gilles; Pecquerie, Laure; Thomas, Yoann; Lefebvre, Sebastien; Le Gendre, Romain; Beliaeff, Benoit; Andréfouët, Serge.
The black-lipped pearl oyster (Pinctada margaritifera) is extensively farmed in French Polynesia to produce black pearls. For a sustainable management of marine resources, studying interactions between organisms and environment, and the associated factors and processes that will impact their life cycle and thus modulate population dynamics is a major research priority. Here, we describe black-lipped pearl oyster energy acquisition and use, and its control by temperature and food concentration within the Dynamic Energy Budget (DEB) theory framework. The model parametrization was based on literature data and a specific laboratory experiment. Model validation was carried out thanks to historical in-situ datasets and a dedicated field survey. Three theoretical...
Tipo: Text Palavras-chave: Bivalve; Physiology; Bioenergetics; Dynamic energy budget theory; Environmental change; Pearl farming.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00613/72469/71614.pdf
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Relations entre l’état d’une ressource et son exploitation via la compréhension et la formalisation des interactions de socio-écosystèmes. Application à la palourde japonaise (Venerupis philippinarum) du bassin d’Arcachon. ArchiMer
Caill-milly, Nathalie.
Significant component of the marine benthic ecosystem, exploited bivalves are mainly located inside the coastal area, which temporal and spatial features generate natural population fluctuations and contribute to considerable biomass and demographic structure changes added to anthropogenic activities’ effects (fishing, pollution,…). Understanding of the relationships between the species, its environment and its exploitation is crucial and their formalization, including for management purposes, requires development of mathematical models that aim at describing the functioning of the system, to explain the observed phenomenon in terms of cause and effect or predict effects of new causes. Originating from venerid culture trials carried out in France in the...
Tipo: Text Palavras-chave: Bivalve; Morphométrie; Allométrie; Dynamique des populations; Système dynamique; Gestion; Palourde japonaise; Venerupis philippinarum; Bivalve; Morphometry; Allometry; Population dynamics; Dynamic system; Management; Manila clam; Venerupis philippinarum.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00139/25034/23143.pdf
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Polyploidy in the Manila clam Ruditapes philippinarum. II. Chemical induction of tetraploid embryos ArchiMer
Diter, Alain; Dufy, Christian.
Tetraploidy was induced in embryos of Ruditapes philippinarum by treating the eggs with cytochalasin B. (CB). Two treatment times were found to be effective in inducing tetraplidy : 0 to 10 minutes after insemination (before formation of the first polar body) and 45 minutes after insemination (just before the first cleavage). Treatments, started 5 and 45 minutes after insemination, produced 64.4 ± 7.8% and 28.3 ± 2.3% tetraploid embrios, respectively, after 6 hours (on the average over 3 experiments). However, no tetraploids were detected in 4-month-old spat. Sperm entry delayed by the CB could be involved in induction of tetraploidy by early treatment.
Tipo: Text Palavras-chave: Ruditapes philippinarum; Bivalve; Tetraploidy; Cytochalasin B.; Ruditapes philippinarum; Bivalve; Tétraploïdie; Cytochalasine B.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/publication-2753.pdf
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Etude cytologique de la gamétogenèse, de la sex-ratio et du cycle de reproduction chez l'huître perlière Pinctada margaritifera (L) var. cumingii (Jameson), (mollusques, bivalves). Comparaison avec le cycle de Pinctada maculata (Gould) ArchiMer
Thielley, Maryse.
The population of Pinctada margaritifera and Pinctada maculata lay eggs throughout the year. Temperature variations induce huge but asynchronous laying. Preferential periods of gonad maturity correspond to the season changings in both species. However. some differences between the two specics are noticed. Both species are protandric hermaphrodites. Female condition is unstable: a reversion to the male condition may be observed and is probably linked with stress. The average size for the first sexual maturation as weil as for sexual conversion are specified in P. margaritifera. The breeding conditions induce modifications in some reproductive cycle processes (maturation, sex-ratio). Some conditions suitable for reproduction may become unfavorable to...
Tipo: Text Palavras-chave: Gamétogenèse; Cycle de reproduction; Sex-ratio; Pinctad margaritifera; Mollusque; Bivalve; Huître perlière; Gametogenesis; Reproductive cycle; Sex-ratio; Pinctada margaritifera; Mollusca; Bivalvia; Pearl oyster.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00440/55208/56677.pdf
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A proteomic study of resistance to Brown Ring disease in the Manila clam, Ruditapes philippinarum ArchiMer
Smits, M.; Artigaud, Sebastien; Bernay, B.; Pichereau, Vianney; Bargelloni, L.; Paillard, Christine.
Marine mollusk aquaculture has more than doubled over the past twenty years, accounting for over 15% of total aquaculture production in 2016. Infectious disease is one of the main limiting factors to the development of mollusk aquaculture, and the difficulties inherent to combating pathogens through antibiotic therapies or disinfection have led to extensive research on host defense mechanisms and host-pathogen relationships. It has become increasingly clear that characterizing the functional profiles of response to a disease is an essential step in understanding resistance mechanisms and moving towards more effective disease control. The Manila clam, Ruditapes philippinarum, is a main cultured bivalve species of economic importance which is affected by...
Tipo: Text Palavras-chave: Bivalve; Diseases; Resistance; Manila clam; Vibrio tapetis; Proteomics; Immune response.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00615/72670/71758.pdf
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Does bivalve mucus favour the growth of their main food source, microalgae? ArchiMer
Cognie, B; Barille, Laurent.
Bivalves, by releasing inorganic nutrients, can stimulate the growth of the primary producers on which they feed. Dissolved excretion and mineralization of biodeposits (pseudofaeces and faeces), two indirect processes related to their feeding activity, have already been identified. This study investigated whether microalgal growth is also dependent on the mucus secreted by bivalves and rejected with their biodeposits. An experimental study based on algal growth tests was conducted to determine whether the mucus coating the pseudofaeces and faeces of the oyster Crassostrea gigas (Thunberg) could stimulate the growth of nine marine microalgae: a Prasinophyceae [Tetraselmis suecica (Butcher)] and eight Diatomophyceae [Amphora sp. (Ehrenberg), Chaetoceros sp....
Tipo: Text Palavras-chave: Bivalve; Microphyte; Mucus; Fèces; Pseudofèces; Bivalve; Microalgae; Mucus; Faeces; Pseudofaeces.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00325/43607/43926.pdf
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Etude expérimentale de différentes conditions de mileu sur le comportement du parasite de la glande digestive de l'huître plate (Ostrea edulis L.) ArchiMer
Comps, Michel.
During trials on the influence of environmental factors on the parasite of the flat oyster digestive gland, we observed that some experimental conditions including the re-immersion after a several day-exsiccation period could result in a decrease of the parasitism rate.
Tipo: Text Palavras-chave: Glande digestive; Huître plate; Ostrea edulis; Bivalve; Pathologie; Parasitologie.
Ano: 1975 URL: http://archimer.ifremer.fr/doc/1975/acte-6168.PDF
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Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure ArchiMer
Lassudrie, Malwenn; Soudant, Philippe; Richard, Gaelle; Henry, N.; Medhioub, Walid; Da Silva, P.m.; Donval, Anne; Bunel, M.; Le Goïc, Nelly; Lambert, Christophe; De Montaudoin, X.; Fabioux, Caroline; Hegaret, Helene.
Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the...
Tipo: Text Palavras-chave: Bivalve; Harmful algal bloom; Oxidative stress; Venerupis philippinarum; Alexandrium ostenfeldii; Perkinsus olseni..
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00189/30022/28507.pdf
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Bilan énergétique chez les mollusques bivalves : terminologie et méthodologie - Compte rendu du groupe de travail réuni au laboratoire national "Ecosystème conchylicole" de l'IFREMER La Tremblade (13 et 14 septembre 1984) ArchiMer
Heral, Maurice; Lucas, Albert; Deslous Paoli, Jean-marc; Goulletquer, Philippe; Masse, Henri; LABEIM (Laboratoire de Biologie et d'Ecologie des Invertébrés Marins).
En septembre 1984, MM. HERAL et DESLOUS-PAOLI organisèrent à La Tremblade, une "réunion méthodologie expérimentale mollusques". Cette rencontre faisait suite à un bref état qui s'était engagé au Congrès de Montpellier sur les "Bases biologiques de l'Aquaculture", où il était apparu que la terminologie et la méthodologie concernant le métabolisme des bivalves devaient être clarifiées. Grâce à une large information auprès des spécialistes français de la physiologie des bivalves, la réunion à La Tremblade a réuni un grand nombre de participants très motivés. Pendant 2 jours des discussions très animées ont permis de bien comprendre les échanges entre les bivalves et leur environnement et d'y jeter un regard neuf, ce qui impliquait une analyse critique du...
Tipo: Text Palavras-chave: Terminologie; Méthodologie; Bilan d'énergie; Physiologie; Bivalve; Terminology; Methodology; Energy budget; Physiology; Bivalve molluscs.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/00014/12562/9432.pdf
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Spatial compartmentalization of free radical formation and mitochondrial heterogeneity in bivalve gills revealed by live-imaging techniques ArchiMer
Rivera-ingraham, Georgina A.; Rocchetta, Iara; Bickmeyer, Ulf; Meyer, Stefanie; Abele, Doris.
Background: Reactive oxygen (ROS) and nitrogen (RNS) species are produced during normal unstressed metabolic activity in aerobic tissues. Most analytical work uses tissue homogenates, and lacks spatial information on the tissue specific sites of actual ROS formation. Live-imaging techniques (LIT) utilize target-specific fluorescent dyes to visualize biochemical processes at cellular level. Results: Together with oxidative stress measurements, here we report application of LIT to bivalve gills for ex-vivo analysis of gill physiology and mapping of ROS and RNS formation in the living tissue. Our results indicate that a) mitochondria located in the basal parts of the epithelial cells close to the blood vessels are hyperpolarized with high Delta Psi m, whereas...
Tipo: Text Palavras-chave: Bivalve; Gill; Live-imaging; Fluorescence; Mitochondria; ROS; RNS.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00626/73808/74412.pdf
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Etude comparative de différents indices de condition chez l'huître creuse (Crassostrea gigas) ArchiMer
Bodoy, Alain; Prou, Jean; Berthome, Jean-paul.
Among the different condition indices, some of them were compared on a population of the japanese oyster, Crassostrea gigas. The follow-up of experimental racks was performed monthly during four years. The use of dry weight in condition indices gave a more precise and more reliable representation for the condition of the oysters than the fresh weight. The sensitivity of these indices, which corresponds to their ability in revealing small changes in the physiological condition, was calculated for each indice. Those exhibiting the best sensitivity were ranked as follow: dry weight on the difference between total weight and shell weight, dry weight on intervalve volume. Other indices including the ratio between dry weight and various assessments of the volume...
Tipo: Text Palavras-chave: Bivalve; Crassostrea gigas; Indice de qualité; Indice de condition; Crassostrea gigas; Bivalve; Condition factor; Condition index.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/00000/2961/9155.pdf
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Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds ArchiMer
Knuckey, R; Brown, M; Robert, Rene; Frampton, D.
A novel technique was developed for the flocculation of marine microalgae commonly used in aquaculture. The process entailed an adjustment of pH of culture to between 10 and 10.6 using NaOH, followed by addition of a non-ionic polymer Magnafloc LT-25 to a final concentration of 0.5 mg L−1. The ensuing flocculate was harvested, and neutralised giving a final concentration factor of between 200- and 800-fold. This process was successfully applied to harvest cells of Chaetoceros calcitrans, C. muelleri, Thalassiosira pseudonana, Attheya septentrionalis, Nitzschia closterium, Skeletonema sp., Tetraselmis suecica and Rhodomonas salina, with efficiencies ≥80%. The process was rapid, simple and inexpensive, and relatively cost neutral with...
Tipo: Text Palavras-chave: Chaetoceros; Thalassiosira; Flocculation; Concentrate; Bivalve; Algal paste.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1820.pdf
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Non‐invasive functional exploration techniques for bivalves with applications to pearl oyster Pinctada margaritifera ArchiMer
Le Moullac, Gilles; Soyez, Claude; Lyonnard, Pierre; Chabrier, Sébastien; Milhade, Leo; Gueguen, Yannick; Beliaeff, Benoit.
Non‐invasive functional exploration techniques can provide information on different aspects of general organism functioning and, unlike lethal or invasive techniques, allow individual organisms to be monitored for as long as necessary. For bivalves, a fairly wide variety of methods and instrumental means exist allowing physiology to be assessed on‐line while keeping animals alive and intact. The current range of non‐invasive techniques for bivalves consists of systems for measuring metabolic flows and valve activity, which can be used on bivalve molluscs for as long as an individual animal's characteristics (e.g. size) make this technically feasible. In this paper, we present some of these non‐invasive techniques with applications for pearl oyster and list...
Tipo: Text Palavras-chave: Bivalve; Filtration; Pearl rotation; Respiration; Valvometry.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00599/71161/69551.pdf
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Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas ArchiMer
Bigot, Laetitia; Zatylny-gaudin, Celine; Rodet, Franck; Bernay, Benoit; Boudry, Pierre; Favrel, Pascal.
Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in species as distant as lophotrochozoans. In this study, two GnRH-related peptides (Cg-GnRH-a and CgGnRH-G) were characterized by mass spectrometry from extracts of the visceral ganglia of the Pacific oyster Crassostrea gigas. These peptides showed a high degree of sequence identity with GnRHs of other mollusks and annelids and to a lesser extent with those of vertebrates or with AKH and corazonins of insects. Both the mature peptides and the transcript encoding the...
Tipo: Text Palavras-chave: Mollusk; Bivalve; Oyster; Neuroendocrinology; Reproduction; GnRH.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00079/19003/17477.pdf
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Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas ArchiMer
Petton, Bruno; De Lorgeril, Julien; Mitta, Guillaume; Daigle, Gaétan; Pernet, Fabrice; Alunno Bruscia, Marianne.
The Pacific oyster Crassostrea gigas is currently being impacted by a polymicrobial disease that involves early viral infection by ostreid herpesvirus-1 (OsHV-1) followed by a secondary bacterial infection leading to death. A widely used method of inducing infection consists of placing specific pathogen-free oysters (‘recipients’) in cohabitation in the laboratory with diseased oysters that were naturally infected in the field (‘donors’). With this method, we evaluated the temporal dynamics of pathogen release in seawater and the cohabitation time necessary for disease transmission and expression. We showed that OsHV-1 and Vibrio spp. in the seawater peaked concomitantly during the first 48 h and decreased thereafter. We found that 1.5 h of cohabitation...
Tipo: Text Palavras-chave: Aquaculture; Bivalve; Epidemiology; Health; Polymicrobial disease; OSHV-1; Pacific oyster mortality syndrome; POMS.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00507/61870/65940.pdf
Registros recuperados: 74
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